#195183 *
Synonyme Bezeichnungen: 4-Phenylspiro[furan-2(3H),1-4-Phenylspiro[furan-2-(3H),1'(3'H)-isobenzofuran]-3,3'-dion; engl., frz. fluorescamine; ital. fluorescammina *
C17H10O4 * Molmasse/F.W.: 278,27 g/mol * CAS-No. [38183-12-9] * EG-/EEC-No.: 253-814-5 (EINECS) * Beilstein Registry number (BRN): 921143 *
Eigenschaften (Literaturwerte)....
Schmelzpunkt/m.p.: 155-156 °C * UV max (Ether): 235, 276, 284, 306 nm (epsilon 25900, 3950, 4100, 3800, ex MI 13);
Typische Verwendungsgebiete: Analytisches Reagenz, als Fluorogen zur fluorimetrischen Bestimmung von primären Aminen, Aminosäuren, Peptiden und Proteinen *
Off-white to yellow powder.
Reagent for fluorescent assay of amino acids, peptides, proteins and other primary amines. Ref. 1,2. Also used for fluorometric assays of some proteolytic enzymes. Ref. 3
Ref.: 1. Weigele, M., et al., J. Am. Chem. Soc. , 94 : 5927 (1972). 2. Udenfriend, S., et al., Science , 178 : 871 (1972). 3. Sogowa, K. and Takahashi, K.J., Biochem. (Tokyo) , 83 : 1783 (1978).
Gefahrenkennung: Xi - REIZEND / IRRITANT *
Literaturhinweise: Non-fluorescent reagent that reacts readily with primary amines to form highly fluorescent compounds: S. Udenfriend, et al., Science 178, 871 (1972); Merck Index 13, 4184; Römpp (9.), S. 1402 (G. Thieme, Stgt. 1995); Anal Biochem 112, 158 (1981); Biochemistry 142, 411 (1984); Methods Enzymol 47, 236 (1977) #38183129
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